Analysis Note

ControlbEND3 cell lysate

Application

Research Sub CategoryOrganelle & Cell MarkersAdhesion (CAMs)

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP of bEND cell lysate (Morgan, 1999).

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in immunhistochemical staining of frozen and paraffin-embedded tissue sections of mouse tongue, heart, uterus, brain, and small intestine (Morgan, 1999).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC of transfected COS-7 cells (Morgan, 1999).

Anti-Endomucin Antibody, clone V.5C7 detects level of Endomucin & has been published & validated for use in WB, IP, IH, FC.

General description

Endomucin is an endothelial sialomucin belonging to a class of glycoproteins characterized by their high levels of O-glycosolation and rich quantities of sialic acid. It inhibits focal adhesion assembly and hinders extracellular matrix and cell interaction. Observed expression is highest in kidney heart, brain, liver, lung, spleen, and thymus. Endomucin is only found in endothelial cells.

Immunogen

Mouse endothelial cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot on mouse endothelioma cell line, bEND3, cell lysate.

Western Blot Analysis: 0.0025 µg/mL of this antibody detected Endomucin in 10 µg of bEND3 cell lysate.

Target description

~ 75kDa observed